Journal: eLife

Article Title: Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A

doi: 10.7554/eLife.69815

Figure Lengend Snippet: ( A ) Images of coronal sections of PND25 mouse brains taken through the anterior and middle portions of the corpus callosum (CC) and stained for neurofilament to label axons and DAPI to stain nuclei. An asterisk indicates the position of the brain midline. Scale bar, 200 μm. ( B ) Quantification of thickness of middle regions of the CC at different points from the brain midline from control (n = 4) and AnkB440 KO (n = 5) brains. ( C ) Images of PND0 brains stained for Satb2, Ctip2, and Tbr1 to label neocortical layers and DAPI to stain nuclei. Scale bar, 200 μm. ( D ) Quantification of total, Sabt2-, Ctip2-, and Tbr1-positive cortical layer thickness from PND0 control (n = 5–6) and AnkB440 KO (n = 4) brains. Data in B and D represent mean ± SEM. Unpaired t test. *p < 0.05, **p < 0.01.

Article Snippet: Commercial antibodies used for immunofluorescence included mouse anti-neurofilament (1:200, clone SMI-312, #837904) from Biolegend; mouse anti-βIII-tubulin (1:100, clone TU-20, #MAB1637), rat anti-L1CAM (1:200, clone 324, #MAB5272), guinea pig anti-NeuN (1:1,000, clone A60, #ABN90P) from Millipore-Sigma; sheep anti-alpha/beta tubulin (1:200, #ATN02) from Cytoskeleton; chicken anti-GFP (1:1000, #GFP-1020) from Aves; rabbit anti-Olig2 (1:200, # 13999–1-AP) from Proteintech, and mouse anti-Satb2 (1:200, clone SATBA4B10, # ab51502), rat anti-Ctip2 (1:500, clone 25B6, # ab18465), rat anti-MBP (1:100, #ab7349) and rabbit anti-Tbr1 (1:200, # ab31940), all from Abcam.

Techniques: Staining

Journal: eLife

Article Title: Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A

doi: 10.7554/eLife.69815

Figure Lengend Snippet:

Article Snippet: Commercial antibodies used for immunofluorescence included mouse anti-neurofilament (1:200, clone SMI-312, #837904) from Biolegend; mouse anti-βIII-tubulin (1:100, clone TU-20, #MAB1637), rat anti-L1CAM (1:200, clone 324, #MAB5272), guinea pig anti-NeuN (1:1,000, clone A60, #ABN90P) from Millipore-Sigma; sheep anti-alpha/beta tubulin (1:200, #ATN02) from Cytoskeleton; chicken anti-GFP (1:1000, #GFP-1020) from Aves; rabbit anti-Olig2 (1:200, # 13999–1-AP) from Proteintech, and mouse anti-Satb2 (1:200, clone SATBA4B10, # ab51502), rat anti-Ctip2 (1:500, clone 25B6, # ab18465), rat anti-MBP (1:100, #ab7349) and rabbit anti-Tbr1 (1:200, # ab31940), all from Abcam.

Techniques: Knock-Out, Recombinant, Mutagenesis, In Situ